Bacteria Classification By Gram Staining

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Term Paper Title	Bacteria Classification By Gram Staining
# of Words	778
# of Pages (250 words per page double spaced)	3.11

Bacteria Classification By Gram Staining

Bacteria Classification By Gram Staining

THE AMERICAN UNIVERSITY IN CAIRO
BIOLOGY DEPARTMENT
SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1
Presented By : Karim A. Zaklama 92-1509 Sci. 453-01
24/2/96

Objective:

     To test a sample of laboratory prepared bacteria and categorise it
according to Christian’s gram positive and gram negative classes and also by
viewing it under a high powered microscope and oil immersions; classify its
shape and note any special characteristics.

Introduction:

     Bacteria was categorised into two groups in 1884 by the Danish
Bacteriologist Christian, gram positive and gram negative by a staining
technique where the ability to avoid de-coloration of  Crystal Violet solution
by alcohol would render the category of gram positive, and gram negative if the
bacteria is de-coloured. This could be noted by the final colour of the
bacteria: a violet colour where Gram positive and a pink colour of the Safranin
added pending the de-colouring process.

Materials:

1.  Bacteria Sample 2.  Microscope Slide 3.  Gram Staining Kit and Wash Bottles
a.  Crystal Violet Solution b.  Iodine Solution c.  95% Ethyl Alcohol d.
Safranin e.  Distilled Water 4.  Bibulous Blotting Paper 5.  Microscope 6.  Oil

Procedure:

A.  Preparation :

1.  Bacteria is cultivated on agar jelly in an incubator at 25°C for 24 hours. 2.
Obtain a microscope slide and with a toothpick, smear a thin coat of the
bacteria sample onto the slide 3.  Cover the smear with a drop Crystal Violet
and leave standing for 20 seconds 4.  Wash off the stain with distilled water;
drain and blot off the excess with bibulous paper. 5.  Apply Gram’s Iodine on
the smear and leave to stand for 1 minute. 6.  Drain the excess iodine and apply
95% Ethyl alcohol for 20 second duration or till the alcohol runs clearly from
the slide. 7.  The smear should rinsed for a few seconds with distilled water to
stop the action of the alcohol. 8.  Drain and blot off the excess with bibulous
9.  Introduce Safranin to the smear and leave standing for 20 seconds. 10.  Wash
off the stain with distilled water; drain and blot off the excess with bibulous
paper. 11.  Leave the slide to air dry.

B.  Examination:

1.  Place the slide under microscope on low powered lens. 2.  Move the slide
using the apparatus until the sample can be seen as a blur under the microscope.
3.  Focus the lens to ensure ...

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