Hormones Regulate Various Aspects Of Development, Growth, And Physiology Of Higher Plants. One Of The Ways That Hormones Can In
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Hormones regulate various aspects of development, growth, and physiology of higher plants. One of the ways that hormones can influence plants is senescence (aging). Our experiment concentrated on the effects of the hormones auxin, cytokinin, and abscisic acid upon. Senescence was calculated by studying the fluctuation of chlorophyll in Triticum aestivum (wheat) leaves. We compared the chlorophyll concentration of the wheat leaves that were treated with the hormones to the concentration of untreated leaves (control). Ten samples each containing ten wheat leaves were treated with the hormone, abscisic acid in different percentages with acetone. The abscisic acid was diluted with acetone to form percentages ranging form 0% to 100%. The first group had 0% abscisic acid, the second 25%, third group 50%, fourth group 75%, and the last grouped was 100% abscisic acid. We tried different proportions of abscisic acid and acetone to see what the hormone does. Using acetone as our blank, we compared the amount of chlorophyll left in the leaves with a spectrophotometer. The spectrophotometer measured the absorbency of the acetone/ chlorophyll extract at 652 nanometers. The hormone, abscisic acid, delayed the aging of the leaves, bred less chlorophyll.
The effect of plant hormones on chlorophyll decomposition in leaf tissue is the subject of study. Senescence, or aging, is a process in plants that is influenced by hormones. The changing color of leaves is a sign that shows the process of decomposition of chlorophyll. Hormones are responsible for the aging of leaves and ultimately the breakdown of chlorophyll. Abscisic acid is a hormone known to inhibit growth of the plant. The hormone should slow the aging of the plant and the decay of chlorophyll(Lewis 576-578). We hypothesized that abscisic acid would promote the aging of plants. We predicted that there would be a declining effect in the chlorophyll concentration in the second week. We concluded that abscisic acid would inhibit plant growth.
Methods As found on pages in this lab manual 16-2 through16-4,(Dickinson college Faculty, 1997).
-Tables and Figures on subsequent pages.
When we soaked the leaves in abscisic acid at different percentages, we observed the following results: 0% abscisic acid had an average of .575 mg chlorophyll/g tissue, 25% abscisic acid had an average of .215 mg chlorophyll/g tissue, 50% abscisic acid had an average of 0.172 mg chlorophy
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